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, 2 (9), 601-8

The DNA Repair Protein rad23 Is a Negative Regulator of Multi-Ubiquitin Chain Assembly

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The DNA Repair Protein rad23 Is a Negative Regulator of Multi-Ubiquitin Chain Assembly

T G Ortolan et al. Nat Cell Biol.

Abstract

Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short multi-Ub chain. The UV sensitivity of rad23Delta was reduced in mutants lacking the E2 enzyme Ubc4, or the multi-Ub chain-promoting factor Ufd2. These studies suggest that the stability of proteolytic substrates is governed by the competing action of multi-Ub chain-promoting and chain-inhibiting factors. The stabilization of DNA repair and stress factors could represent an important biological function of Rad23.

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