ICSBP directs bipotential myeloid progenitor cells to differentiate into mature macrophages

Immunity. 2000 Aug;13(2):155-65. doi: 10.1016/s1074-7613(00)00016-9.


During hematopoiesis, myeloid progenitor cells give rise to granulocytes and macrophages. To study the role for ICSBP, a hematopoietic cell-specific transcription factor in myeloid cell development, the gene was introduced into myeloid progenitor cells established from ICSBP-/- mice. ICSBP retrovirus-transduced cells differentiated into mature macrophages with phagocytic activity, which coincided with the induction of specific target DNA binding activity. Similar to macrophages in vivo, ICSBP-transduced cells were growth arrested, expressed many macrophage-specific genes, and responded to macrophage activation signals. Contrary to this, ICSBP transducion led to repression of granulocyte-specific genes and inhibited G-CSF-mediated granulocytic differentiation in these and other myeloid progenitor cells. Together, ICSBP has a key role in the myeloid cell lineage selection and macrophage maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Gene Expression Regulation
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / physiology*
  • Interferon Regulatory Factors
  • Interferons / physiology
  • Leukopoiesis
  • Macrophages / cytology*
  • Macrophages / physiology*
  • Mice
  • Repressor Proteins / physiology*


  • Interferon Regulatory Factors
  • Repressor Proteins
  • interferon regulatory factor-8
  • Interferons