Enteroendocrine localization of GLP-2 receptor expression in humans and rodents

Gastroenterology. 2000 Sep;119(3):744-55. doi: 10.1053/gast.2000.16489.


Background & aims: Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R), but the cellular targets for the diverse actions of GLP-2 remain unknown.

Methods: GLP-2R expression in rodent and human tissues was examined using a combination of Northern blotting, reverse-transcription polymerase chain reaction (RT-PCR), and immunocytochemistry.

Results: A single major GLP-2R messenger RNA transcript was detected by Northern blot analysis in rodent stomach, duodenum, jejunum, ileum, and colon, but not in rodent esophagus. GLP-2R expression was also detected by RT-PCR in RNA from the hypothalamus, brain stem, and lung. Immunocytochemical localization of human GLP-2R expression using specific antisera detected GLP-2R immunopositivity in subsets of endocrine cell populations in the epithelium of the stomach and both the small and large bowel.

Conclusions: These findings suggest that enteroendocrine-derived GLP-2 acts directly on endocrine cells to induce one or more downstream mediators of GLP-2 action in the gastrointestinal tract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Brain Stem / metabolism
  • Cells, Cultured
  • Digestive System / metabolism
  • Endocrine Glands / metabolism*
  • Glucagon-Like Peptide-1 Receptor
  • Humans
  • Hypothalamus / metabolism
  • Immunohistochemistry
  • Intestinal Mucosa / metabolism*
  • Lung / metabolism
  • Mice
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Glucagon / genetics
  • Receptors, Glucagon / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution


  • GLP1R protein, human
  • Glp1r protein, mouse
  • Glp1r protein, rat
  • Glucagon-Like Peptide-1 Receptor
  • RNA, Messenger
  • Receptors, Glucagon