lazarus is a novel pbx gene that globally mediates hox gene function in zebrafish

Mol Cell. 2000 Aug;6(2):255-67. doi: 10.1016/s1097-2765(00)00027-7.

Abstract

Individual vertebrate Hox genes specify aspects of segment identity along the anterior-posterior axis. The exquisite in vivo specificity of Hox proteins is thought to result from their interactions with members of the Pbx/Exd family of homeodomain proteins. Here, we report the identification and cloning of a zebrafish gene, lazarus, which is required globally for segmental patterning in the hindbrain and anterior trunk. We show that lazarus is a novel pbx gene and provide evidence that it is the primary pbx gene required for the functions of multiple hox genes during zebrafish development. lazarus plays a critical role in orchestrating the corresponding segmentation of the hindbrain and the pharyngeal arches, a key step in the development of the vertebrate body plan.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning / genetics*
  • DNA-Binding Proteins / genetics
  • Embryo, Nonmammalian / physiology
  • Gene Expression Regulation*
  • Genes, Homeobox*
  • Genes, Regulator*
  • Mutagenesis
  • Polymerase Chain Reaction
  • Pre-B-Cell Leukemia Transcription Factor 1
  • Proto-Oncogene Proteins / genetics
  • Rhombencephalon / embryology*
  • Zebrafish / embryology*
  • Zebrafish / genetics*

Substances

  • DNA-Binding Proteins
  • Pre-B-Cell Leukemia Transcription Factor 1
  • Proto-Oncogene Proteins
  • pbx1 protein, human