Abstract
We report the cloning and sequencing of the glnN gene encoding a class III glutamine synthetase from the cyanobacterium Synechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression of glnN is impaired in NtcA- and P(II)-deficient mutants. The only parameter which was negatively affected in the glnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Base Sequence
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Cyanobacteria / enzymology*
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Cyanobacteria / genetics
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DNA, Bacterial
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DNA-Binding Proteins / genetics
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Gene Expression Regulation, Bacterial
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Glutamate-Ammonia Ligase / genetics
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Glutamate-Ammonia Ligase / metabolism*
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Molecular Sequence Data
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Mutagenesis
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Nitrogen / metabolism*
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PII Nitrogen Regulatory Proteins
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Time Factors
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Transcription Factors / genetics
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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PII Nitrogen Regulatory Proteins
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Transcription Factors
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ntcA protein, Synechococcus
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PIID regulatory protein, Bacteria
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Glutamate-Ammonia Ligase
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Nitrogen