Expression and immunolocalization of multidrug resistance protein 2 in rabbit small intestine

Eur J Pharmacol. 2000 Jul 21;400(2-3):195-8. doi: 10.1016/s0014-2999(00)00391-5.


Multidrug resistance protein 2 (MRP2) is an ATP-dependent transporter of anionic drugs and conjugates. It functions as an efflux pump in the apical membranes of liver and kidney cells, but its membrane localization in small intestine has not yet been defined. The present study demonstrates exclusive localization of Mrp2 to the brush-border (apical) membrane of villi, decreasing in intensity from the villus tip to the crypts. In immunoblot analysis of crude membranes of various rabbit tissues, Mrp2 was only found in small intestine, kidney and liver. These results are in-line with the supposed function of Mrp2 in drug excretion.

MeSH terms

  • Animals
  • Anion Transport Proteins
  • Antibodies / immunology
  • Antibody Specificity
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / immunology
  • Duodenum / metabolism
  • Duodenum / ultrastructure
  • Fluorescent Antibody Technique, Indirect
  • Guinea Pigs
  • Immunoblotting
  • Intestine, Small / metabolism*
  • Intestine, Small / ultrastructure
  • Microvilli / metabolism
  • Rabbits
  • Rats


  • Anion Transport Proteins
  • Antibodies
  • Carrier Proteins