Transgenic methods for increasing Drosophila life span

Mech Ageing Dev. 2000 Sep 1;118(1-2):1-14. doi: 10.1016/s0047-6374(00)00152-4.


At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in ability to meet several technical challenges, and the relative advantages and disadvantages of each are discussed. Using these techniques, over-expression of the hsp70, Cu/ZnSOD and MnSOD genes has each been demonstrated to increase Drosophila life span.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Aging / genetics
  • Animals
  • Animals, Genetically Modified
  • Drosophila / genetics*
  • Drosophila / physiology*
  • Genetic Techniques
  • HSP70 Heat-Shock Proteins / genetics
  • Longevity / genetics*
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Superoxide Dismutase / genetics


  • HSP70 Heat-Shock Proteins
  • Superoxide Dismutase