Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. Summer 2000;6(2):111-7.
doi: 10.1089/107662900419410.

Evidence of an Efflux Pump in Serratia Marcescens

Affiliations

Evidence of an Efflux Pump in Serratia Marcescens

M Berlanga et al. Microb Drug Resist. .

Abstract

Spontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10(-7) to 10(-9). Active expulsion of antibiotic was explored as a possible mechanism of resistance in mutants as well as changes in topoisomerase target genes. The role of extrusion mechanisms in determining the emergence of multidrug-resistant bacteria was also examined. Mutants resistant to high concentrations of fluoroquinolones had a single mutation in their gyrA QRDR sequences, whereas the moderate resistance in the rest of mutants was due to extrusion of the drug.

Similar articles

See all similar articles

Cited by 2 articles

Publication types

MeSH terms

LinkOut - more resources

Feedback