Upregulation of interleukin-8 and polarized epithelial expression of interleukin-8 receptor A in ovarian carcinomas

Acta Obstet Gynecol Scand. 2000 Sep;79(9):777-84.


Background: The chemokine interleukin-8 is present in a variety of tumor types with suggested effects on proliferation, migration, and angiogenesis. Elevated levels of interleukin-8 are present in cyst fluids from malignant ovarian tumors. The origin and potential targets for this chemokine in ovarian tumors were investigated in this study.

Methods: Interleukin-8 and its receptors were analyzed in 26 ovarian samples, including both normal and neoplastic tissue, with immunohistochemistry, Western blotting, and in situ hybridization.

Results: The mRNA for IL-8 was detected in higher amounts in the epithelial compartments compared to stromal areas, while the IL-8 protein was present in both epithelial and stromal areas, and in cystic formations of the tumors. The tissue levels of IL-8 protein increased with lower differentiation of the tumors. Both types of IL-8 receptors were detected in most specimens. A typical expression pattern for IL-8 receptor A was detected, with expression only on the luminal side of the epithelial tumor cells, while IL-8 receptor B was more evenly distributed in the tissue.

Conclusions: An increased synthesis of IL-8 during dedifferentiation of the tumor, and a typical expression pattern of the IL-8 receptor A were detected, indicating a function for IL-8 in biology of epithelial ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism*
  • Blotting, Western
  • Carcinoma / immunology*
  • Carcinoma / pathology*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism*
  • Ovarian Neoplasms / immunology*
  • Ovarian Neoplasms / pathology*
  • RNA, Messenger / metabolism
  • Receptors, Interleukin / metabolism*
  • Receptors, Interleukin-8A
  • Up-Regulation


  • Antigens, CD
  • Interleukin-8
  • RNA, Messenger
  • Receptors, Interleukin
  • Receptors, Interleukin-8A