Preliminary communication. MRNA expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts cultured on several matrix proteins and ivory surfaces

Biosci Biotechnol Biochem. 2000 Aug;64(8):1771-3. doi: 10.1271/bbb.64.1771.

Abstract

We demonstrated that the transcriptional expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts were regulated by different matrix proteins that bind to integrin on osteoclast, such as collagen type I (CoI), fibronectin (FN), vitronectin (VN), osteopontin (OPN), and ivory. Results suggested that the OPN-integrin alphavbeta3 binding plays a more important role than CoI-alpha2beta1 binding in the regulation of osteoclast activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins*
  • Cathepsin K
  • Cathepsins / biosynthesis*
  • Cathepsins / genetics
  • Cells, Cultured
  • Collagen / metabolism
  • Extracellular Matrix Proteins / metabolism*
  • Fibronectins / metabolism
  • Matrix Metalloproteinase 9 / biosynthesis*
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases / biosynthesis*
  • Metalloendopeptidases / genetics
  • Osteoclasts / enzymology*
  • Osteopontin
  • RNA, Messenger / biosynthesis*
  • RNA-Binding Proteins / biosynthesis*
  • RNA-Binding Proteins / genetics
  • Rabbits
  • Receptors, Vitronectin / metabolism
  • Sialoglycoproteins / metabolism
  • Surface Properties
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics
  • Transcription, Genetic
  • Vitronectin / metabolism

Substances

  • Bacterial Proteins
  • Extracellular Matrix Proteins
  • Fibronectins
  • MtrB protein, Bacteria
  • RNA, Messenger
  • RNA-Binding Proteins
  • Receptors, Vitronectin
  • Sialoglycoproteins
  • Transcription Factors
  • Vitronectin
  • Osteopontin
  • Collagen
  • Cathepsins
  • Cathepsin K
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases
  • Matrix Metalloproteinase 9