Determination of total taurine in pet foods by liquid chromatography of the dansyl derivative: collaborative study

J AOAC Int. 2000 Jul-Aug;83(4):784-8.

Abstract

A liquid chromatographic (LC) method for the determination of total taurine in pet foods was evaluated in a collaborative study. Ten laboratories assayed 6 blind duplicate pairs of wet and dry pet foods. The taurine in the 6 sample pairs ranged from low (170 mg/kg) to high (2250 mg/kg) concentrations as is. Collaborators also assayed a sample of known taurine concentration for familiarization purposes. Samples were hydrolyzed to release bound taurine, which was subsequently converted to the dansyl derivative and quantitated by gradient-elution LC with fluorescence detection. Repeatability relative standard deviations, RSDr, ranged from 3.2 to 10.0%; reproducibility relative standard deviations, RSDR, ranged from 6.1 to 16.1%. The method has been adopted Official First Action status by AOAC INTERNATIONAL.

MeSH terms

  • Animals
  • Animals, Domestic*
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / statistics & numerical data
  • Dansyl Compounds*
  • Food Analysis / methods*
  • Hydrolysis
  • Laboratories
  • Linear Models
  • Reproducibility of Results
  • Taurine / analysis*

Substances

  • Dansyl Compounds
  • Taurine