Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, Wasabi

Mech Ageing Dev. 2000 Jul 31;116(2-3):125-34. doi: 10.1016/s0047-6374(00)00114-7.


6-Methylsulfinylhexyl isothiocyanate (MS-ITC) was isolated from wasabi (Wasabia japonica, Japanese domestic horseradish) as a potential inhibitor of human platelet aggregation in vitro through our extensive screening of vegetables and fruits. In the course of an another screening for the induction of glutathione S-transferase (GST) activity in RL34 cells, MS-ITC was inadvertently isolated from wasabi as a potential inducer of GST. MS-ITC administered to rats or mice also showed both activities in vivo. As a result from elucidation of the platelet aggregation inhibition and the GST induction mechanisms of MS-ITC, the isothiocyanate moiety of MS-ITC plays an important role for antiplatelet and anticancer activities because of its high reactivity with sulfhydryl (RSH) groups in biomolecules (GSH, cysteine residue in a certain protein, etc.).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Brassicaceae / chemistry*
  • Enzyme Induction
  • Glutathione Transferase / biosynthesis
  • Humans
  • Isothiocyanates / chemistry
  • Isothiocyanates / isolation & purification*
  • Isothiocyanates / pharmacology
  • Japan
  • Mice
  • Molecular Structure
  • Platelet Aggregation Inhibitors / chemistry
  • Platelet Aggregation Inhibitors / isolation & purification*
  • Platelet Aggregation Inhibitors / pharmacology
  • Rabbits
  • Rats
  • Structure-Activity Relationship
  • Vegetables


  • 6-methylsulfinylhexyl isothiocyanate
  • Antineoplastic Agents, Phytogenic
  • Isothiocyanates
  • Platelet Aggregation Inhibitors
  • Glutathione Transferase