Modulation of collagen-induced arthritis by IL-4 and dexamethasone: the synergistic effect of IL-4 and dexamethasone on the resolution of CIA

Immunopharmacology. 2000 Sep;49(3):317-24. doi: 10.1016/s0162-3109(00)00248-4.


We investigated effects of IL-4, dexamethasone (DXM), and the combination of IL-4 and DXM, low- or high-dose, on collagen-induced arthritis (CIA) in DBA/1 mice and correlated severity of arthritis with changes in IL-10 and IFN-gamma. Compared with control mice, mice treated with IL-4 had increased IL-10 with the same degree of arthritis, whereas mice treated with high-dose DXM had decreased IL-10 and increased IFN-gamma production with less severe arthritis. Mice treated with low-dose DXM showed the absence of IL-10 and increased IFN-gamma production with a trend toward the resolution of arthritis. Mice treated with IL-4 and low-dose DXM had neither IL-10 nor IFN-gamma production but revealed less severe arthritis, compared with mice treated with low-dose DXM alone. These results suggest that the beneficial effects of high-dose DXM and the combination of IL-4 and DXM on CIA are independent of IL-10 and IFN-gamma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Arthritis, Experimental / immunology*
  • Arthritis, Experimental / prevention & control*
  • Cattle
  • Collagen / immunology*
  • Cytokines / biosynthesis
  • Dexamethasone / administration & dosage
  • Dexamethasone / pharmacology*
  • Dose-Response Relationship, Immunologic
  • Drug Combinations
  • Drug Synergism
  • Injections, Intradermal
  • Injections, Intraperitoneal
  • Interleukin-4 / administration & dosage
  • Interleukin-4 / pharmacology*
  • Male
  • Mice
  • Mice, Inbred DBA
  • Severity of Illness Index


  • Adjuvants, Immunologic
  • Cytokines
  • Drug Combinations
  • Interleukin-4
  • Dexamethasone
  • Collagen