Schizosaccharomyces pombe Rad9 contains a BH3-like region and interacts with the anti-apoptotic protein Bcl-2

FEBS Lett. 2000 Sep 15;481(2):122-6. doi: 10.1016/s0014-5793(00)01975-x.


Here we report that the Schizosaccharomyces pombe Rad9 (SpRad9) protein contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. These observations suggest that SpRad9 may represent the first member of the Bcl-2 protein family identified in yeast, though the cell death pathways in S. pombe may differ from those found in mammals.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Apoptosis* / drug effects
  • Binding Sites
  • Cell Cycle Proteins / chemistry*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Division / drug effects
  • Cell Line
  • Conserved Sequence / genetics
  • DNA Damage / drug effects
  • DNA Damage / genetics
  • Evolution, Molecular
  • Gene Deletion
  • Gene Expression
  • Genes, Suppressor / genetics
  • Humans
  • Methyl Methanesulfonate / pharmacology
  • Mutagens / pharmacology
  • Protein Binding
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Schizosaccharomyces* / cytology
  • Schizosaccharomyces* / drug effects
  • Schizosaccharomyces* / genetics
  • Thiamine / pharmacology
  • Two-Hybrid System Techniques


  • Cell Cycle Proteins
  • Mutagens
  • Proto-Oncogene Proteins c-bcl-2
  • rad9 protein
  • Methyl Methanesulfonate
  • Thiamine