Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate

AIDS. 2000 Sep 8;14(13):1917-20. doi: 10.1097/00002030-200009080-00006.


Objective: To characterize functional properties of HIV-specific IgA in samples representing both systemic and mucosal compartments of HIV-1 highly exposed persistently seronegative (HEPS) individuals.

Methods: IgA was purified from plasma and mucosal samples from HEPS individuals and tested for the ability to neutralize infection of peripheral blood mononuclear cells (PBMC) by a non-syncytium inducing HIV-1 (clade B) primary isolate. None of these individuals had measurable HIV-1-specific IgG.

Results: HIV-1-specific neutralizing activity of the purified IgA from plasma (n = 15), saliva (n = 15) and cervicovaginal fluid (CVF) (n = 14) were found in the majority of samples (73, 73 and 79%, respectively). In contrast, plasma, saliva and CVF samples of low-risk, uninfected HIV-seronegative individuals lacked neutralizing IgA, with the exception of two out of 34 (6%) saliva samples.

Conclusion: Mucosal and plasma IgA from HEPS individuals can neutralize HIV-1 infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cervix Uteri / immunology
  • Female
  • HIV Infections / virology
  • HIV Seronegativity / immunology*
  • HIV-1 / immunology*
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin A / immunology*
  • Immunoglobulin A, Secretory / immunology*
  • Mucous Membrane / immunology
  • Neutralization Tests
  • Saliva / immunology
  • Sex Work
  • Vagina / immunology


  • Immunoglobulin A
  • Immunoglobulin A, Secretory