Helicobacter pylori inhibits phagocytosis by professional phagocytes involving type IV secretion components

Mol Microbiol. 2000 Sep;37(6):1389-404. doi: 10.1046/j.1365-2958.2000.02089.x.


Gastric infections by Helicobacter pylori are characteristically associated with an intense inflammation and infiltration of mainly polymorphonuclear lymphocytes (PMNs) and monocytes. The inflammatory response by infiltrated immune cells appears to be a primary cause of the damage to surface epithelial layers and may eventually result in gastritis, peptic ulcer, gastric cancer and/or MALT-associated gastric lymphoma. Our analysis of the interaction between H. pylori and PMNs and monocytes revealed that H. pylori inhibits its own uptake by these professional phagocytes. To some degree, this effect resembles antiphagocytosis by Yersinia enterocolitica. Increasing numbers of bacteria associated per cell are more efficient at blocking their own engulfment. In H. pylori, bacterial protein synthesis is necessary to block phagocytic uptake, as shown by the time and concentration dependence of the bacteriostatic protein synthesis inhibitor chloramphenicol. Furthermore, H. pylori appears broadly to inhibit the phagocytic function of monocytes and PMNs, as infection with H. pylori abrogates the phagocytes' ability to engulf latex beads or adherent Neisseria gonorrhoeae cells. This antiphagocytic phenotype depends on distinct virulence (vir) genes, such as virB7 and virB11, encoding core components of a putative type IV secretion apparatus. Our data indicate that H. pylori exhibits an antiphagocytic activity that may play an essential role in the immune escape of this persistent pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / drug effects
  • Bacterial Proteins / genetics
  • Blood Group Antigens
  • Chloramphenicol / pharmacology
  • Helicobacter pylori / drug effects
  • Helicobacter pylori / metabolism*
  • Helicobacter pylori / pathogenicity*
  • Humans
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Monocytes / microbiology
  • Mutation
  • Neutrophils / microbiology
  • Phagocytes / microbiology*
  • Phagocytosis / physiology*
  • Protein Synthesis Inhibitors / pharmacology
  • Species Specificity
  • Virulence / genetics
  • Virulence Factors*


  • Bacterial Proteins
  • Blood Group Antigens
  • Protein Synthesis Inhibitors
  • Virulence Factors
  • Chloramphenicol