Prevalence of non-O157:H7 Shiga Toxin-Producing Escherichia Coli in Diarrheal Stool Samples From Nebraska

Emerg Infect Dis. Sep-Oct 2000;6(5):530-3. doi: 10.3201/eid0605.000513.

Abstract

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.

MeSH terms

  • Diarrhea / epidemiology*
  • Diarrhea / microbiology*
  • Escherichia coli O157 / classification*
  • Escherichia coli O157 / metabolism
  • Escherichia coli O157 / pathogenicity
  • Feces / microbiology
  • Humans
  • Immunoenzyme Techniques
  • Nebraska / epidemiology
  • Polymerase Chain Reaction
  • Prevalence
  • Serotyping / methods*
  • Shiga Toxin / biosynthesis
  • Shiga Toxin / isolation & purification*

Substances

  • Shiga Toxin