Structure of succinoglycan from an infectious strain of Agrobacterium radiobacter

Int J Biol Macromol. 2000 Aug 28;27(5):319-26. doi: 10.1016/s0141-8130(00)00131-8.

Abstract

The exopolysaccharide produced by a cystic fibrosis clinical isolate of Agrobacterium radiobacter was shown by monosaccharide and methylation analyses, degradation with succinoglycanase and NMR analysis to be a succinoglycan with the structure shown below. (S)-pyruvic acid is found stoichiometrically as 4,6-O-ketal substituent of terminal glucose. Succinic acid is present in 40% of the repeating units and it is attached to O-6 of the 3-linked glucose next to the pyruvate carrying sugar. Some evidence is found that a small amount of succinic acid (ca. 6% of the total) is linked to O-6 of another undetermined glucose. [structure: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Carbon Radioisotopes
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Polysaccharides, Bacterial / chemistry*
  • Pyruvic Acid / metabolism
  • Rhizobium / chemistry*
  • Succinates / metabolism

Substances

  • Carbon Radioisotopes
  • Polysaccharides, Bacterial
  • Succinates
  • succinoglycan
  • Pyruvic Acid