Assessment of fresh and stored duck spermatozoa quality via in vitro sperm-egg interaction assay

Theriogenology. 2000 Jul 15;54(2):283-90. doi: 10.1016/s0093-691x(00)00348-4.


An in vitro sperm-egg interaction assay was used to measue the quality of duck spermatozoa in fresh and stored semen. The inner perivitelline layer (IPVL), which had been separated from laid duck eggs, was incubated with spermatozoa in vitro. The number of points of sperm hydrolysis in the IPVL in vitro was logarithmically correlated with the fertility of the eggs laid by inseminated females, for both fresh semen (r = 0.85, P < 0.001) and stored semen at 5 degrees C for 24 h (r = 0.84, P < 0.001). After semen storage, the ability of spermatozoa to hydrolyze the IPVL decreased by 67.4% compared with the values for fresh semen, whereas egg fertility and sperm motility decreased by 47.8% and 15.2%, respectively. These results suggest that the in vitro sperm-egg interaction assay accurately reflects the fertilizing ability of fresh and stored duck spermatozoa and detects spermatozoal damage due to semen storage more sensitively than motility or fertility tests.

MeSH terms

  • Animals
  • Ducks / physiology*
  • Female
  • Fertility / physiology*
  • Indicators and Reagents / chemistry
  • Male
  • Microscopy, Phase-Contrast / veterinary
  • Ovum / chemistry
  • Ovum / physiology
  • Random Allocation
  • Semen Preservation / veterinary
  • Sperm Motility / physiology*
  • Sperm-Ovum Interactions / physiology*
  • Spermatozoa / physiology*
  • Sulfhydryl Compounds / chemistry
  • Vitelline Membrane / physiology


  • Indicators and Reagents
  • Sulfhydryl Compounds
  • thioacetic acid