Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model

J Trauma. 2000 Sep;49(3):511-4. doi: 10.1097/00005373-200009000-00020.

Abstract

Objective: The purpose of the study was to determine whether electrolyzed oxidized water (EOW) functions as a bactericide in burn injury with Pseudomonas aeruginosa infection in a rat burn-wound model.

Methods: Anesthetized Sprague-Dawley rats (n = 31) were subjected to third-degree burns to 30% of total body surface area. Two days after injury, all rats were infected with P. aeruginosa using 1 mL of a suspension containing 1 x 10(8) colony-forming units. Rats were assigned to one of three groups: no irrigation (group I), irrigation with physiologic saline (group II), or irrigation with EOW (group III). Blood culture, endotoxin levels, and survival rates were determined.

Results: Survival rate was significantly higher in group III than in groups I or II (p < 0.0001). Serum endotoxin levels on day 3 after infection in group III were significantly lower than the levels in group I (p < 0.01) and group II (p < 0.01). There were significant differences between the three groups in the culture of P. aeruginosa (p < 0.05).

Conclusion: Irrigation and disinfection with EOW may become useful in preventing burn-wound sepsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burns / complications*
  • Disease Models, Animal
  • Disinfectants / therapeutic use*
  • Disinfection
  • Electrolysis
  • Male
  • Oxidation-Reduction
  • Pseudomonas Infections / complications
  • Pseudomonas Infections / prevention & control*
  • Rats
  • Rats, Sprague-Dawley
  • Therapeutic Irrigation
  • Water / chemistry*
  • Wound Infection / complications
  • Wound Infection / prevention & control*

Substances

  • Disinfectants
  • Water