Levels of house-dust-mite allergen in homes of nonallergic people in Pavia, Italy

Allergy. 2000 Sep;55(9):873-8. doi: 10.1034/j.1398-9995.2000.00584.x.


Background: HDM distribution varies between geographic areas and may be affected by housing characteristics. We quantified Der p 1 and Der f 1 and assessed the relationships between their levels and housing characteristics in homes of nonallergic subjects.

Methods: Der f 1 and Der p 1 were measured by ELISA in dust samples from living-room floors and mattresses of 44 homes of nonallergic subjects in Pavia. Information about household characteristics was obtained by questionnaire.

Results: Der p 1 and Der f 1 concentrations (microg/g dust, median) were 0.34 and 7.8 on mattresses, and 0.15 and 0.83 on living-room floors. Higher Der f 1 levels on mattresses were associated with synthetic pillows (P<0.05), and (only when expressed as microg/m2) with bedding washing temperature of < or = 60 degrees C (P<0.05). Der f 1 levels were higher on the living-room floors of homes located on lower (< or =first) floors (P<0.05). Good correlations were found between Der p 1 and Der f 1 expressed as microg/g dust and microg/m2 on both mattresses and living-room floors.

Conclusions: In homes of nonallergic subjects in northern Italy, Der f 1 exceeded the threshold for sensitization in a high proportion of mattress samples.

MeSH terms

  • Animals
  • Antigens, Dermatophagoides
  • Beds
  • Dust / analysis*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Floors and Floorcoverings
  • Glycoproteins / analysis*
  • Housing*
  • Humans
  • Italy
  • Mites / immunology*


  • Antigens, Dermatophagoides
  • Dust
  • Glycoproteins