Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells

Biochim Biophys Acta. 2000 Jun 21;1492(1):127-38. doi: 10.1016/s0167-4781(00)00094-4.


The expression of human purinergic P2 receptors (P2X1-7 and P2Y1-11) as well as the ecto-enzymes apyrase (CD39) and 5'-nucleotidase (CD73) was investigated on the nucleic acid level during granulocytic and monocytic differentiation of HL60 cells and on peripheral human blood leukocytes. RT-PCR and dot-blot hybridization assays indicated that mRNA transcripts of all analyzed P2 receptors apart from the P2X3 receptor were expressed during myeloid development of HL60 cells, showing a distinct regulation during the course of differentiation. In blood leukocytes, transcripts of P2X5, P2X7 and all P2Y receptors, except for P2Y6, receptor were found. CD39 and CD73 showed a marked upregulation during myeloid maturation. Functional analysis of P2 receptor-mediated intracellular Ca(2+)-increase after stimulation with ATP revealed no change during granulocytic differentiation, but showed a strong attenuation in both potency and efficacy during monocytic development of HL60 cells.

MeSH terms

  • 5'-Nucleotidase / biosynthesis
  • 5'-Nucleotidase / genetics
  • Adenosine Triphosphatases*
  • Adenosine Triphosphate / metabolism
  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics
  • Apyrase
  • Calcium / metabolism
  • Cell Differentiation / genetics*
  • Cell Differentiation / physiology
  • Gene Expression*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • HL-60 Cells
  • Humans
  • Immunoblotting
  • RNA, Messenger / biosynthesis
  • Receptors, Purinergic P2 / biosynthesis
  • Receptors, Purinergic P2 / genetics*
  • Receptors, Purinergic P2X
  • Receptors, Purinergic P2X7
  • Receptors, Purinergic P2Y1


  • Antigens, CD
  • P2RX7 protein, human
  • P2RY1 protein, human
  • P2RY11 protein, human
  • RNA, Messenger
  • Receptors, Purinergic P2
  • Receptors, Purinergic P2X
  • Receptors, Purinergic P2X7
  • Receptors, Purinergic P2Y1
  • Adenosine Triphosphate
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • 5'-Nucleotidase
  • Adenosine Triphosphatases
  • Apyrase
  • CD39 antigen
  • Calcium