Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L

Biochim Biophys Acta. 2000 Jun 21;1492(1):211-5. doi: 10.1016/s0167-4781(00)00073-7.

Abstract

Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA, Complementary / analysis
  • DNA, Plant / analysis
  • Molecular Sequence Data
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Plants, Toxic*
  • Protease Inhibitors*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Tobacco / genetics*
  • Tobacco / virology
  • Tobacco Mosaic Virus

Substances

  • DNA, Complementary
  • DNA, Plant
  • Plant Proteins
  • Protease Inhibitors
  • proteinase inhibitor II protein, plant

Associated data

  • GENBANK/AF205851
  • GENBANK/AF205852