Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3(1)

Biochim Biophys Acta. 2000 Aug 24;1487(1):106-11.

Abstract

A cotton Ltp3 gene and its 5' and 3' flanking regions have been cloned with a PCR-based genomic DNA walking method. The amplified 2.6 kb DNA fragment contains sequences corresponding to GH3 cDNA which has been shown to encode a lipid transfer protein (LTP3). The gene has an intron of 80 bp which is located in the region corresponding to the C-terminus of LTP3. The Ltp3 promoter was systematically analyzed in transgenic tobacco plants by employing the Escherichia coli beta-glucuronidase gene (GUS) as a reporter. The results of histochemical and fluorogenic GUS assays indicate that the 5' flanking region of the Ltp3 gene contains cis-elements conferring the trichome specific activity of Ltp3 promoter.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Plant
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / chemistry
  • Genes, Plant*
  • Gossypium / genetics*
  • Molecular Sequence Data
  • Plant Proteins
  • Plants, Toxic
  • Promoter Regions, Genetic*
  • Textiles
  • Tobacco / genetics

Substances

  • Antigens, Plant
  • Carrier Proteins
  • DNA, Complementary
  • Plant Proteins
  • lipid transfer proteins, plant

Associated data

  • GENBANK/AF228333