Global role for chromatin remodeling enzymes in mitotic gene expression

Cell. 2000 Sep 1;102(5):587-98. doi: 10.1016/s0092-8674(00)00081-7.

Abstract

Regulation of eukaryotic gene expression requires ATP-dependent chromatin remodeling enzymes, such as SWI/SNF, and histone acetyltransferases, such as Gcn5p. Here we show that SWI/SNF remodeling controls recruitment of Gcn5p HAT activity to many genes in late mitosis and that these chromatin remodeling enzymes play a role in regulating mitotic exit. In contrast, interphase expression of GAL1, HIS3, PHO5, and PHO8 is accompanied by SWI/SNF-independent recruitment of Gcn5p HAT activity. Surprisingly, prearresting cells in late mitosis imposes a requirement for SWI/SNF in recruiting Gcn5p HAT activity to the GAL1 promoter, and GAL1 expression also becomes dependent on both chromatin remodeling enzymes. We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylation
  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism
  • Anaphase / drug effects
  • Cell Cycle Proteins*
  • Chromatin / chemistry
  • Chromatin / enzymology
  • Chromatin / genetics
  • Chromatin / metabolism*
  • DNA-Binding Proteins*
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Deletion
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal / genetics
  • Histone Acetyltransferases
  • Histones / chemistry
  • Histones / metabolism
  • Interphase / drug effects
  • Mitosis* / drug effects
  • Mitosis* / genetics
  • Nocodazole / pharmacology
  • Nucleic Acid Conformation
  • Phenotype
  • Promoter Regions, Genetic / genetics
  • Protein Kinases / chemistry
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Saccharomyces cerevisiae* / cytology
  • Saccharomyces cerevisiae* / enzymology
  • Saccharomyces cerevisiae* / genetics
  • Saccharomyces cerevisiae* / metabolism
  • Time Factors
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Cell Cycle Proteins
  • Chromatin
  • DNA-Binding Proteins
  • Fungal Proteins
  • Histones
  • SWI5 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Acetyltransferases
  • Histone Acetyltransferases
  • Protein Kinases
  • SNF1-related protein kinases
  • Protein-Serine-Threonine Kinases
  • Nocodazole