Molecular structure of human TFIIH

Cell. 2000 Sep 1;102(5):599-607. doi: 10.1016/s0092-8674(00)00082-9.

Abstract

TFIIH is a multiprotein complex required for both transcription and DNA repair. Single particles of human TFIIH were revealed by electron microscopy and image processing at a resolution of 3.8 nm. TFIIH is 16 x 12.5 x 7.5 nm in size and is organized into a ring-like structure from which a large protein domain protrudes out. A subcomplex assembled from five recombinant core subunits also forms a circular architecture that can be superimposed on the ring found in human TFIIH. Immunolabeling experiments localize several subunits: p44, within the ring structure, forms the base of the protruding protein density which includes the cdk7 kinase, cyclin H, and MAT1. Within the ring structure, p44 was flanked on either side by the XPB and XPD helicases. These observations provide us with a quartenary organizational model of TFIIH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cyclin H
  • Cyclin-Dependent Kinases*
  • Cyclins / chemistry
  • Cyclins / metabolism
  • Cyclins / ultrastructure
  • DNA Helicases / chemistry*
  • DNA Helicases / metabolism
  • DNA Helicases / ultrastructure*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / ultrastructure
  • HeLa Cells
  • Humans
  • Image Processing, Computer-Assisted
  • Macromolecular Substances
  • Microscopy, Immunoelectron
  • Models, Molecular
  • Multiprotein Complexes
  • Protein Structure, Quaternary
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / metabolism
  • Protein-Serine-Threonine Kinases / ultrastructure
  • Proteins / chemistry
  • Proteins / metabolism
  • Proteins / ultrastructure
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / ultrastructure
  • Transcription Factor TFIIH
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism
  • Transcription Factors / ultrastructure*
  • Transcription Factors, TFII*
  • Transcription, Genetic
  • Xeroderma Pigmentosum Group D Protein

Substances

  • Antibodies, Monoclonal
  • CCNH protein, human
  • Cyclin H
  • Cyclins
  • DNA-Binding Proteins
  • Macromolecular Substances
  • Multiprotein Complexes
  • Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • Transcription Factors
  • Transcription Factors, TFII
  • XPBC-ERCC-3 protein
  • Transcription Factor TFIIH
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • cyclin-dependent kinase-activating kinase
  • DNA Helicases
  • Xeroderma Pigmentosum Group D Protein
  • ERCC2 protein, human