CENP-G in neocentromeres and inactive centromeres

Chromosoma. 2000;109(5):328-33. doi: 10.1007/s004120000082.


CENP-G is a novel constitutive centromere-specific protein localized to the kinetochore inner plate and subjacent region. It has been identified as associating specifically with the alpha-1 subfamily of alpha-satellite DNA. In the present work, the localization of CENP-G was compared with that of other CENPs by immunofluorescence and fluorescence in situ hybridization. Studies were carried out on four abnormal human centromeres: two neocentromeres and two inactive centromeres. CENP-G was detected in one of the two inactive centromeres but not in the other that shows a partial deletion of the alphoid DNA. Interestingly, CENP-G is also present in neocentromeres, which lack alphoid DNA sequences, and in the human Y chromosome, which lacks the alpha-1 type of satellite DNA. These data provide further evidence that CENP-G may be an essential factor in centromeric function and that in centromeres lacking the alpha-1 subfamily of alphoid DNA, other DNA sequences are able to bind CENP-G.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Centromere / chemistry*
  • Child, Preschool
  • Chromosomal Proteins, Non-Histone / isolation & purification*
  • Chromosome Aberrations*
  • Cytogenetics
  • DNA-Binding Proteins / isolation & purification*
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence


  • CENP-G protein, human
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins