Study of the B cell memory compartment in common variable immunodeficiency

Eur J Immunol. 2000 Sep;30(9):2516-20. doi: 10.1002/1521-4141(200009)30:9<2516::AID-IMMU2516>3.0.CO;2-Z.


We evaluated the B cell memory pool among blood B cells from 20 patients with common variable immunodeficiency (CVID). CD27+ B cell number was normal or increased in 6 patients (with 95% CD27+ B cells in 1 patient) and decreased in 14 patients. In 13 or 15 patients studied, the CD27 molecule was detectable on less than 50% IgG or IgA B cells, indicating a defect in the maturation of these memory cells. Within the group of patients with a low number of CD27+ B cells, no up-regulation of this molecule was observed after in vitro stimulation of purified B cells from 3 of 5 patients studied, suggesting an intrinsic B cell defect. In addition, ligation of the CD27 molecule was unable to trigger terminal differentiation of purified B cells in 1 of 2 cases with a large number of CD27+ B cells. Finally, the CD27 ligand was normally expressed on activated T cells in only 5 of 14 patients studied. These data confirm the heterogeneity of immunological defects in patients with CVID. Abnormal expression and/or function of the CD27-CD70 members of the TNF/TNF receptor family contribute to the immunological defect.

MeSH terms

  • Antigens, CD*
  • B-Lymphocytes / immunology*
  • CD27 Ligand
  • CD3 Complex / immunology
  • Cell Differentiation
  • Common Variable Immunodeficiency / immunology*
  • Humans
  • Immunologic Memory*
  • Lymphocyte Activation
  • Membrane Proteins / analysis*
  • T-Lymphocytes / immunology
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / analysis*


  • Antigens, CD
  • CD27 Ligand
  • CD3 Complex
  • CD70 protein, human
  • Membrane Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7