Time-course Changes in the Expression of Na+, K+-ATPase in Gills and Pyloric Caeca of Brown Trout (Salmo Trutta) During Acclimation to Seawater

Physiol Biochem Zool. Jul-Aug 2000;73(4):446-53. doi: 10.1086/317737.

Abstract

Changes in protein and mRNA expression of Na(+),K(+)-ATPase in gills and pyloric caeca of brown trout were investigated on a detailed time course after transfer from freshwater to 25 ppt seawater (SW). A transient deflection in plasma osmolality and muscle water content lasting from 4 h until day 3 was followed by restoration of hydromineral balance from day 5 onward. Gills and pyloric caeca responded to SW transfer by increasing Na(+),K(+)-ATPase activity from days 5 and 3, respectively, onward. In both tissues, this response was preceded by an increase in alpha-subunit Na(+), K(+)-ATPase mRNA as early as 12 h posttransfer. The similarity of the response in these two organs suggests that they both play significant physiological roles in restoring hydromineral balance after abrupt increase in salinity. Further, SW transfer induced a slight, though significant, increase in primary gill filament Na(+), K(+)-ATPase immunoreactive (NKIR) cell abundance. This was paralleled by a marked (50%) decrease in secondary lamellar NKIR cell abundance after less than 1 d in SW. Thus, SW acclimation in brown trout is characterised by a lasting decrease in overall NKIR cell abundance in the gill. We propose that SW transfer stimulates Na(+),K(+)-ATPase enzymatic activity within individual chloride cells long before (<1 d) it becomes apparent in measurements of whole-gill homogenate enzymatic activity. This is supported by the early stabilisation (12 h) of hydromineral balance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Water / metabolism
  • Female
  • Gills / enzymology*
  • Male
  • Muscles / metabolism
  • Pylorus / enzymology*
  • RNA, Messenger / metabolism
  • Salmon / blood
  • Salmon / metabolism*
  • Sodium-Potassium-Exchanging ATPase
  • Water-Electrolyte Balance

Substances

  • RNA, Messenger
  • Sodium-Potassium-Exchanging ATPase