Packaging of small molecules into VP1-virus-like particles of the human polyomavirus JC virus

J Virol Methods. 2000 Oct;90(1):85-90. doi: 10.1016/s0166-0934(00)00226-3.


Recombinantly expressed VP1-virus-like particles (VP1-VLP) of human polyomavirus JC virus (JCV) were described recently as a new DNA transporter system. It was shown that DNA molecules could be packaged into VP1-VLP during a controlled chemical reassociation/dissociation process. In the present study VP1-VLP were studied as carriers for pharmaceutical substances. Propidium iodide (PI) was packaged into VP1-VLP as a reporter molecule. The PI-containing VP1-VLP could be detected directly by flow cytometry. The fluorescence intensity of the VP1-VLP depended strongly on the initial PI concentration. This packaging method is easy to handle and applicable to viruses and VP1-VLP which can be dissociated and reassociated chemically.

MeSH terms

  • Capsid / genetics
  • Capsid / isolation & purification
  • Capsid / metabolism*
  • Capsid Proteins*
  • DNA / genetics
  • DNA / metabolism*
  • Drug Delivery Systems
  • Flow Cytometry
  • Humans
  • JC Virus / metabolism*
  • Microscopy, Electron
  • Propidium / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Virion / genetics
  • Virion / metabolism*
  • Virus Assembly*


  • Capsid Proteins
  • Recombinant Fusion Proteins
  • VP1 protein, polyomavirus
  • Propidium
  • DNA