Eosinophil granule-derived major basic protein induces IL-8 expression in human intestinal myofibroblasts

Clin Exp Immunol. 2000 Oct;122(1):35-40. doi: 10.1046/j.1365-2249.2000.01337.x.


Eosinophil infiltration occurs in a variety of allergic and inflammatory diseases. The release of preformed mediators from eosinophils may contribute to inflammatory responses. We investigated the ability of eosinophil-derived major basic protein and eosinophil-derived neurotoxin to stimulate production of IL-8 from intestinal myofibroblasts. Intestinal myofibroblasts (18-Co cells) were incubated with major basic protein, eosinophil-derived neurotoxin, or a synthetic analogue of major basic protein, poly-L-arginine. Immunoreactive IL-8 was measured by ELISA and IL-8 mRNA levels were analysed by Northern blot or reverse transcription-polymerase chain assay. Major basic protein induced IL-8 mRNA production and release of significant levels of IL-8 immunoreactive protein. By contrast, eosinophil-derived neurotoxin stimulated little IL-8 release. The induction of IL-8 mRNA by poly-L-arginine was significantly inhibited by actinomycin D. These findings demonstrate a novel interaction between eosinophils and intestinal fibroblasts that may be involved in the pathogenesis of diseases associated with tissue eosinophilia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood Proteins / isolation & purification
  • Blood Proteins / pharmacology*
  • Cells, Cultured
  • Eosinophil Granule Proteins
  • Eosinophil-Derived Neurotoxin
  • Eosinophils / cytology
  • Eosinophils / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Gene Expression / drug effects
  • Humans
  • Interleukin-8 / biosynthesis*
  • Interleukin-8 / genetics
  • Intestines / cytology
  • Muscle, Smooth / cytology
  • Peptides / pharmacology
  • Proteins / pharmacology
  • Recombinant Proteins / pharmacology
  • Ribonucleases*
  • Tumor Necrosis Factor-alpha / pharmacology


  • Blood Proteins
  • Eosinophil Granule Proteins
  • Interleukin-8
  • Peptides
  • Proteins
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • polyarginine
  • Eosinophil-Derived Neurotoxin
  • Ribonucleases