Antigen-induced IL-17 response in the peripheral blood mononuclear cells (PBMC) of healthy controls

Clin Exp Immunol. 2000 Oct;122(1):41-8. doi: 10.1046/j.1365-2249.2000.01328.x.


IL-17 is a T cell cytokine with a complex and important role in the immune system. It has been detected in rheumatoid arthritis (RA) synovial membrane and found to stimulate the production of the proinflammatory cytokines IL-6, IL-8, tumour necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro. To date, there are few data available on the agents that stimulate IL-17 production. We therefore investigated the in vitro IL-17 response to a variety of mitogens and antigens, and compared the IL-17 response to interferon-gamma (IFN-gamma), IL-4, IL-10 and TNF-alpha. In this study we used a type-0 antigen, tetanus toxoid (TT), a type-1 antigen, PPD from Mycobacterium tuberculosis, a potential type-2 rye grass (RG) antigen (Lol I) and an autoantigen SS.B (La), to stimulate PBMC from healthy controls. Cytokine mRNA was measured using semiquantitative reverse transcriptase-polymerase chain reaction and cytokine protein measured using specific ELISA techniques, while the frequency of IL-17-producing T cells was determined by flow cytometry. The mitogens concanavalin A, phytohaemagglutinin and phorbol myristate acetate/ionomycin induced a significant increase in IL-17, with the highest levels being produced by anti-CD3/anti-CD28 stimulation. The antigens TT and PPD significantly increased IL-17 mRNA expression over time, but failed to have such an effect at the protein level. IL-17 protein was also detectable in both antigen-specific (TT, SS. B) and non-specific T cell clones, but at levels lower than IFN-gamma. IL-17 production did not correlate with either the type-1 cytokine IFN-gamma or TNF-alpha or the type-2 cytokine IL-4 or IL-10 at either the mRNA or protein level.

MeSH terms

  • Adult
  • Aged
  • Allergens*
  • Antigens, Plant
  • Cell Division / drug effects
  • Cells, Cultured
  • Gene Expression / drug effects
  • Health Status
  • Humans
  • Interleukin-17 / genetics*
  • Interleukin-17 / metabolism
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology*
  • Middle Aged
  • Mitogens / pharmacology
  • Plant Proteins / immunology*
  • Plant Proteins / pharmacology
  • RNA, Messenger
  • Tetanus Toxoid / immunology*
  • Tetanus Toxoid / pharmacology
  • Tuberculin / immunology*
  • Tuberculin / pharmacology


  • Allergens
  • Antigens, Plant
  • Interleukin-17
  • Lol p I protein, Lolium perenne
  • Mitogens
  • Plant Proteins
  • RNA, Messenger
  • Tetanus Toxoid
  • Tuberculin