Analysis of PCP-data to Determine the Fraction of Cells in the Various Phases of Cell Cycle

Radiat Environ Biophys. 1975 Jun 13;12(1):31-9. doi: 10.1007/BF02339807.

Abstract

Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and (G2 + M)-phase are obtained. The methods are applied to PCP-measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bone Marrow Cells*
  • Carcinoma, Ehrlich Tumor / metabolism
  • Carcinoma, Ehrlich Tumor / pathology*
  • Cell Division*
  • Cytological Techniques
  • DNA / biosynthesis
  • Humans
  • L Cells / cytology*
  • L Cells / metabolism
  • Mathematics
  • Pulse Radiolysis

Substances

  • DNA