Abstract
FKS1 and FKS2 encode alternative catalytic subunits of the glucan synthases that are responsible for synthesis of beta-1,3-glucan in the Saccharomyces cerevisiae cell wall. Disruption of FKS1 reduces the glucan content of the cell wall, increases chitin content and activates the expression of CWP1, which encodes a glycosylphosphatidylinositol (GPI)-dependent cell wall protein. These cellular responses have been regarded as compensating for cell wall damage in order to maintain cell wall integrity. Here, we report the identification, by genome-wide screening, of 22 genes that are transcriptionally up-regulated in fks1delta cells. Among them, five genes were found to encode GPI-attached proteins, three of which are covalently associated with the cell wall. Deletion and replacement analysis of the promoter regions identified Rlm1-binding sequences as being responsible for the up-regulation following disruption of FKS1. Using the rlm1delta tetOp-FKS1 strain, in which the expression of FKS1 can be repressed by doxycycline, we examined the requirement for Rlm1 for the transcriptional up-regulation of these five genes. Three of the five genes were not up-regulated by doxycycline, indicating that Rlm1 mediates their up-regulation when FKS1 is inactivated. The remaining two genes were up-regulated by doxycycline, suggesting that a transcription factor other than Rlm1 is involved in their response to disruption of FKS1.
MeSH terms
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Aspartic Acid Endopeptidases / genetics
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Aspartic Acid Endopeptidases / metabolism
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Cell Wall / genetics
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Cytochrome c Group / genetics
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Cytochrome c Group / metabolism
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Cytochromes c*
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Echinocandins
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Profiling / methods
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Gene Expression Regulation, Fungal
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Glucosyltransferases*
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Glycoside Hydrolases / genetics
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Glycosylphosphatidylinositols / metabolism*
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Histone Deacetylases
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MADS Domain Proteins
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism*
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Deletion
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Up-Regulation
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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CWP1 protein, S cerevisiae
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CYC1 protein, S cerevisiae
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Cytochrome c Group
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Echinocandins
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Fungal Proteins
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Glycosylphosphatidylinositols
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MADS Domain Proteins
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Membrane Glycoproteins
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Membrane Proteins
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RLM1 protein, S cerevisiae
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Recombinant Fusion Proteins
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Repressor Proteins
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SIN3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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YLR194C protein, S cerevisiae
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Cytochromes c
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Glucosyltransferases
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FKS1 protein, S cerevisiae
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CRH1 protein, S cerevisiae
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Glycoside Hydrolases
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beta-Galactosidase
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Aspartic Acid Endopeptidases
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YPS3 protein, S cerevisiae
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Histone Deacetylases