Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis

FEBS Lett. 2000 Sep 29;482(1-2):37-43. doi: 10.1016/s0014-5793(00)02019-6.


We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP-1. XCIRP-1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP-1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP-1 specific as they could be rescued by sense transcript. Suppression of XCIRP-1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP-1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim-1 and WT1 genes. These results suggest that XCIRP-1 is required for the specification and morphogenetic lineage migration of the pronephros.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abnormalities, Multiple / genetics
  • Animals
  • Cell Division
  • Cloning, Molecular
  • Embryo, Nonmammalian / physiology
  • Evolution, Molecular
  • Female
  • Kidney / cytology
  • Kidney / embryology*
  • Morphogenesis
  • Phenotype
  • Phylogeny
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Suppression, Genetic
  • Transcription, Genetic
  • Xenopus Proteins*
  • Xenopus laevis / embryology*
  • Xenopus laevis / genetics


  • CIRP2 protein, Xenopus
  • Protein Isoforms
  • RNA-Binding Proteins
  • Xenopus Proteins