A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography

Biochemistry. 1975 Oct 21;14(21):4634-8. doi: 10.1021/bi00692a011.


An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA nucleotidyltransferase, EC] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography
  • Chromatography, Gel
  • DNA-Directed RNA Polymerases / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology*
  • Polyethyleneimine


  • Polyethyleneimine
  • DNA-Directed RNA Polymerases