Clarithromycin resistance stability in Helicobacter pylori: influence of the MIC and type of mutation in the 23S rRNA

J Antimicrob Chemother. 2000 Oct;46(4):613-6. doi: 10.1093/jac/46.4.613.

Abstract

Thirty clarithromycin-resistant Helicobacter pylori strains (MIC range 8-64 mg/L) were subcultured in a drug-free medium and the MIC was determined every five passages to detect in vitro stability of resistance. Three out of the 30 (10%) lost their resistance after 10, 13 or 18 subcultures (MIC decrease from 8 to 0.008, from 16 to 0. 064 and from 32 to 0.016 mg/L). The effect of four macrolides at subinhibitory concentrations on the development of resistance was studied in H. pylori NCTC 11638 and TIGR 26695. A change in the MIC was observed only when NCTC11638 was exposed to 0.5 x MIC of erythromycin for 20 days.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Cells, Cultured
  • Clarithromycin / pharmacology*
  • Culture Media
  • Drug Resistance, Microbial / genetics
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / genetics
  • Humans
  • Microbial Sensitivity Tests / methods
  • Mutation / drug effects*
  • Mutation / genetics
  • RNA, Ribosomal, 23S / drug effects*
  • RNA, Ribosomal, 23S / genetics

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • RNA, Ribosomal, 23S
  • Clarithromycin