A new positive/negative selectable marker, puDeltatk, for use in embryonic stem cells

Genesis. 2000 Sep;28(1):31-5. doi: 10.1002/1526-968x(200009)28:1<31::aid-gene40>3.0.co;2-k.

Abstract

A novel positive/negative selection cassette, puDeltatk, was generated. pu(Delta)tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (DeltaTk). Murine embryonic stem (ES) cells transfected with pu(Delta)tk become resistant to puromycin and sensitive to 1-(-2-deoxy-2-fluoro-1-beta-D-arabino-furanosyl)-5-iodouracil (FIAU). Unlike other HSV1 tk transgenes, puDeltatk is readily transmitted through the male germ line. Thus pu(Delta)tk is a convenient positive/negative selectable marker that can be widely used in many ES cell applications.

MeSH terms

  • Acetyltransferases / genetics*
  • Animals
  • Artificial Gene Fusion / methods
  • Cells, Cultured
  • Clone Cells / enzymology
  • Drug Resistance / genetics
  • Electroporation
  • Embryo, Mammalian / cytology*
  • Embryo, Mammalian / enzymology*
  • Genetic Markers
  • Herpesvirus 1, Human / enzymology
  • Herpesvirus 1, Human / genetics
  • Male
  • Mice
  • Phosphoglycerate Kinase / genetics
  • Stem Cells / enzymology*
  • Thymidine Kinase / genetics*
  • Viral Proteins / genetics

Substances

  • Genetic Markers
  • Viral Proteins
  • Acetyltransferases
  • puromycin N-acetyltransferase
  • Thymidine Kinase
  • Phosphoglycerate Kinase