A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants

Science. 2000 Oct 6;290(5489):142-4. doi: 10.1126/science.290.5489.142.

Abstract

Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Amino Acid Sequence
  • Cysteine Endopeptidases / metabolism*
  • Gene Silencing*
  • Genes, Plant
  • Green Fluorescent Proteins
  • Luminescent Proteins / genetics
  • Molecular Sequence Data
  • Nicotiana / genetics*
  • Nicotiana / metabolism
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Tumors / genetics
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Plasmids
  • Potexvirus / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Transcription, Genetic
  • Transgenes
  • Viral Proteins / metabolism*

Substances

  • Luminescent Proteins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • Viral Proteins
  • rgs-CaM protein, Nicotiana tabacum
  • Green Fluorescent Proteins
  • Cysteine Endopeptidases
  • HC-Pro protein, potyvirus