Abstract
Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Agrobacterium tumefaciens / genetics
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Amino Acid Sequence
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Cysteine Endopeptidases / metabolism*
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Gene Silencing*
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Genes, Plant
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Molecular Sequence Data
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Nicotiana / genetics*
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Nicotiana / metabolism
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plant Tumors / genetics
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Plants, Genetically Modified
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Plants, Toxic*
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Plasmids
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Potexvirus / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Transcription, Genetic
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Transgenes
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Viral Proteins / metabolism*
Substances
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Luminescent Proteins
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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Viral Proteins
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rgs-CaM protein, Nicotiana tabacum
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Green Fluorescent Proteins
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Cysteine Endopeptidases
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HC-Pro protein, potyvirus