Prevalence of human papillomavirus DNA in different histological subtypes of cervical adenocarcinoma

Am J Pathol. 2000 Oct;157(4):1055-62. doi: 10.1016/S0002-9440(10)64619-6.


The prevalence of human papilloma virus (HPV) DNA in different histological subtypes of cervical adenocarcinoma and related tumors was examined using formalin-fixed, paraffin-embedded tissue samples from 105 primary cervical adenocarcinomas and adenosquamous carcinomas. Broad-spectrum HPV DNA amplification and genotyping was performed with the SPF10 primer set and line probe assay (LiPA), respectively. HPV DNA was detected in 82 of 90 (91%) mucinous adenocarcinomas, encompassing endocervical, intestinal, and endometrioid histological subtypes, and in nine of nine adenosquamous tumors (100%). HPV DNA was not detected in any nonmucinous adenocarcinomas including clear cell, serous, and mesonephric carcinomas (0/6). The most common viral types detected in adenocarcinoma were HPV 16 (50%) and HPV 18 (40%), followed by HPV 45 (10%), HPV52 (2%), and HPV 35 (1%). Multiple HPV types were detected in 9.7% of the cases. In conclusion, mucinous adenocarcinomas and adenosquamous carcinomas of the cervix demonstrate a very high prevalence of HPV DNA, similar to that reported for cervical squamous cell carcinoma. Only rare histological variants of cervical adenocarcinoma seem unrelated to HPV infection.

MeSH terms

  • Adenocarcinoma / pathology*
  • Adenocarcinoma / virology*
  • Adenocarcinoma, Mucinous / pathology
  • Adenocarcinoma, Mucinous / virology
  • Adult
  • Carcinoma, Adenosquamous / pathology
  • Carcinoma, Adenosquamous / virology
  • DNA, Viral / analysis*
  • Female
  • Humans
  • Middle Aged
  • Papillomaviridae / genetics*
  • Prevalence
  • Uterine Cervical Neoplasms / pathology*
  • Uterine Cervical Neoplasms / virology*


  • DNA, Viral