2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure?

Cell Biol Int. 2000;24(10):757-60. doi: 10.1006/cbir.2000.0556.


Intracellular 2,7-dichlorofluorescin (H(2)DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H(2)DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that increased rate of H(2)DCF oxidation be interpreted as an indication of general oxidative stress due to a variety of reasons, including depletion of antioxidants, rather than as a specific proof of augmented ROS formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzyme Inhibitors / pharmacology
  • Ethylmaleimide / pharmacology
  • Fluoresceins / metabolism*
  • Glutathione / metabolism*
  • Glutathione / pharmacology
  • Hydrogen Peroxide / pharmacology
  • Oxidants / pharmacology
  • Oxidation-Reduction
  • Reactive Oxygen Species / physiology*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism


  • 2',7'-dichlorodihydrofluorescein diacetate
  • Enzyme Inhibitors
  • Fluoresceins
  • Oxidants
  • Reactive Oxygen Species
  • Hydrogen Peroxide
  • Glutathione
  • Ethylmaleimide