Solution structure of the TAZ2 (CH3) domain of the transcriptional adaptor protein CBP

J Mol Biol. 2000 Oct 20;303(2):243-53. doi: 10.1006/jmbi.2000.4141.


The TAZ2 (CH3) domain of the transcriptional adapter protein CBP has been implicated in direct functional interactions with numerous cellular transcription factors and viral oncoproteins. The solution structure of the TAZ2 domain of murine CBP has been determined by nuclear magnetic resonance (NMR). The protein adopts a novel helical fold stabilized by three zinc ions, each of which is bound to one histidine and three cysteine ligands in HCCC-type motifs. Each zinc-binding site is formed from the carboxy terminus of an alpha-helix, a short loop, and the amino terminus of the next alpha-helix. A peptide derived from the N-terminal transactivation domain of p53 binds specifically to one face of the TAZ2 domain. The close similarities between the TAZ2 and TAZ1 (CH1 domain of CBP/p300) sequences suggest that both domains will adopt similar three-dimensional structures.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus E1A Proteins / metabolism
  • Amino Acid Sequence
  • Animals
  • Apoproteins / chemistry
  • Apoproteins / metabolism
  • Binding Sites
  • CREB-Binding Protein
  • Circular Dichroism
  • Cysteine / metabolism
  • Histidine / metabolism
  • Ligands
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular*
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Solutions
  • Thermodynamics
  • Trans-Activators / chemistry*
  • Trans-Activators / metabolism*
  • Transcriptional Activation
  • Tumor Suppressor Protein p53 / chemistry
  • Tumor Suppressor Protein p53 / metabolism
  • Zinc / metabolism
  • Zinc Fingers*


  • Adenovirus E1A Proteins
  • Apoproteins
  • Ligands
  • Nuclear Proteins
  • Recombinant Proteins
  • Solutions
  • Trans-Activators
  • Tumor Suppressor Protein p53
  • Histidine
  • CREB-Binding Protein
  • Crebbp protein, mouse
  • Zinc
  • Cysteine