Human beta defensin is absent in burn blister fluid

Burns. 2000 Dec;26(8):724-6. doi: 10.1016/s0305-4179(00)00052-8.

Abstract

Defensins are a family of cationic antimicrobial peptides that participate in innate host defence. Human beta defensin-2 (HBD-2) is produced by human keratinocytes, and has a potent bactericidal activity against a wide spectrum of microorganisms. We have recently shown that expression of HBD-2 is present in normal skin and lost in the full-thickness burn wound. Defensins have been found in the blister fluid of chronic wounds. Our study was designed to examine blister fluid from partial-thickness burns for defensin content. Fluid from five patients was collected from partial-thickness burn blisters, and then analysed by sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a monoclonal antibody and rabbit polyclonal antibody to HBD-2. The assay was validated against a Western blot assay for HBD-2 in samples of bronchoalveolar lavage fluid from patients with inflammatory lung disease. No HBD-2 was detectable in any of the burn blister fluids analysed. HBD-2 is lost in the full-thickness burn wound, and we have now demonstrated its absence in burn blister fluid. This finding represents evidence of a host defence defect within the burn wound and suggests a possible therapeutic role for antimicrobial peptides in the management of burn wounds.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Aged
  • Aged, 80 and over
  • Blister / immunology
  • Blister / metabolism*
  • Blotting, Western
  • Burns / immunology
  • Burns / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Exudates and Transudates / chemistry*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • Wound Healing / immunology
  • Wound Healing / physiology
  • beta-Defensins / analysis*

Substances

  • beta-Defensins