Rapamycin (AY-22,989), a new antifungal antibiotic. II. Fermentation, isolation and characterization

J Antibiot (Tokyo). 1975 Oct;28(10):727-32. doi: 10.7164/antibiotics.28.727.


Rapamycin is a new antifungal antibiotic produced by Streptomyces hygroscopicus NRRL 5491. It was isolated from the mycelium by solvent extraction, purified by silica gel column chromatography and crystallized as a colorless solid which melts at 183 approximately to 185 degrees C and has the empirical formula C56H89NO14. From its characteristic ultraviolet absorption spectrum rapamycin can be classified as a triene. It is highly active against various Candida species, especially Candida albicans. Its activity is compared with that of amphotericin B, candicidin and nystatin.

MeSH terms

  • Amphotericin B / pharmacology
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology
  • Bacteriological Techniques
  • Candicidin / pharmacology
  • Candida / drug effects
  • Candida albicans / drug effects
  • Fermentation
  • Nystatin / pharmacology
  • Polyenes / isolation & purification
  • Polyenes / pharmacology
  • Streptomyces / analysis*


  • Antifungal Agents
  • Polyenes
  • Nystatin
  • Candicidin
  • Amphotericin B