Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 124 (2), 1007-18

Mycoplasma Hominis: Growth, Reproduction, and Isolation of Small Viable Cells

Mycoplasma Hominis: Growth, Reproduction, and Isolation of Small Viable Cells

J Robertson et al. J Bacteriol.

Abstract

Improved methods for studying the growth of Mycoplasma hominis (ATCC 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. Cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. Similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefore seems characteristic of the species. The pleomorphic populations contain small forms having diameters within the 100- to 250-nm size range reported for "elementary bodies." Such forms were isolated from this strain of M. hominis by sequential filtration using gravity alone, after cell aggregates were dispersed by Pronase treatment. Of the small bodies which traversed membranes of 220-nm pore size, a negligible number grew in liquid or on solid media, suggesting that these were not essential reproductive units in a life cycle, but involution forms due to growth in an altered environment.

Similar articles

See all similar articles

Cited by 7 PubMed Central articles

See all "Cited by" articles

References

    1. J Bacteriol. 1973 Nov;116(2):1011-8 - PubMed
    1. Z Med Mikrobiol Immunol. 1970;155(3):248-74 - PubMed
    1. J Bacteriol. 1962 May;83:948-55 - PubMed
    1. Virology. 1963 Mar;19:250-60 - PubMed
    1. J Bacteriol. 1963 Jan;85(1):134-6 - PubMed

LinkOut - more resources

Feedback