New neuropeptides containing carboxy-terminal RFamide and their receptor in mammals

Nat Cell Biol. 2000 Oct;2(10):703-8. doi: 10.1038/35036326.


Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically respond to synthetic hRFRP-1 and hRFRP-3 but not to hRFRP-2. RFRP and OT7T022 mRNAs are expressed in particular regions of the rat hypothalamus, and intracerebroventricular administration of hRFRP-1 increases prolactin secretion in rats. Our results indicate that a variety of RFamide-related peptides may exist and function in mammals.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cloning, Molecular
  • Humans
  • Mice
  • Molecular Sequence Data
  • Neuropeptides / chemistry
  • Neuropeptides / genetics
  • Neuropeptides / isolation & purification*
  • Rats
  • Receptors, Neuropeptide / genetics
  • Receptors, Neuropeptide / isolation & purification*
  • Sequence Homology, Amino Acid
  • Species Specificity


  • Neuropeptides
  • RFamide peptide
  • Receptors, Neuropeptide
  • arginylphenylalaninamide

Associated data

  • GENBANK/AB040103
  • GENBANK/AB040104
  • GENBANK/AB040288
  • GENBANK/AB040289
  • GENBANK/AB040290
  • GENBANK/AB040291