Lipid peroxidation as determined by plasma isoprostanes is related to disease severity in mild asthma

Lipids. 2000 Sep;35(9):967-74. doi: 10.1007/s11745-000-0607-x.


Oxidlative stress is believed to play an important role in the pathophysiology of asthma. Recently discovered F2-isoprostanes, of which 8-iso-PGF2alpha is the most well-known isomer, have emerged as the most reliable marker of in vivo oxidative stress. The aim of this study was to examine 8-iso-PGF2alpha as a biomarker of oxidative stress in mild asthma in relation to endogenous and dietary antioxidant protection. Total (free and esterified) plasma 8-iso-PGF2alpha, plasma dietary antioxidants (vitamins E and C, Beta-carotene, Zn, and Se), and erythrocyte antioxidant enzyme activities (glutathione peroxidase and superoxide dismutase) were measured in 15 mild asthmatics and 15 age-and sex-matched controls. Total plasma 8-iso-PGF2alpha levels [median (quartile 1 - quartile 3)] were significantly increased in the asthmatics [213 pg/mL (122-455) vs. 139 pg/mL (109-174), P= 0.042]. The 8-iso PGF2alpha levels were found to be associated with clinical asthma severity (P = 0.044) and inhaled corticosteroid use (P = 0.027) in asthmatics. No differences were observed in the plasma dietary antioxidant vitamins. The asthmatics had significantly lower plasma levels of Zn (P = 0.027) and Se (P = 0.006). Plasma Se correlated negatively with 8-iso-PGF2alpha (r = -0.725, P= 0.002). No differences between the groups were observed for glutathione peroxidase or superoxide dismutase, however, superoxide dismutase activity was negatively associated with asthma severity (P = 0.042). In conclusion, oxidative stress is increased in mildly asthmatics, as reflected by increased plasma levels of 8-iso-PGF2alpha and a deficiency in plasma Zn and Se. The isoprostane 8-iso-PGF2alpha may provide a useful tool in intervention studies aimed at improving clinical status in asthma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antioxidants / analysis
  • Antioxidants / metabolism*
  • Asthma / blood*
  • Asthma / enzymology
  • Asthma / metabolism
  • Asthma / physiopathology*
  • Biomarkers / blood
  • Blood Cell Count
  • Child
  • Diet
  • Dinoprost / analogs & derivatives*
  • Dinoprost / blood
  • Dinoprost / metabolism
  • Erythrocytes / enzymology
  • Erythrocytes / metabolism
  • F2-Isoprostanes
  • Female
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lipid Peroxidation*
  • Male
  • Metals / blood
  • Oxidative Stress*
  • Phenotype
  • Superoxide Dismutase / metabolism
  • Vitamins / blood
  • Vitamins / metabolism


  • Antioxidants
  • Biomarkers
  • F2-Isoprostanes
  • Metals
  • Vitamins
  • 8-epi-prostaglandin F2alpha
  • Dinoprost
  • Glutathione Peroxidase
  • Superoxide Dismutase