Identification of a novel human mitochondrial D-loop RNA species which exhibits upregulated expression following cellular immortalization

Biochem Biophys Res Commun. 2000 Sep 24;276(2):439-46. doi: 10.1006/bbrc.2000.3501.


We report the identification and characterization of a novel human mitochondrial RNA species approximately 0.47 kb long that is transcribed from the mtDNA L-strand and is derived from the D-loop. Its expression increases when human cells become immortal, a key event in tumorigenesis. The RNA is therefore designated IDL (Immortalization-associated D-Loop). Sequence and hybrid cell analyses suggest that the increased level of IDL RNA in immortal cells is due to a recessive change, possibly in the activity of a trans-acting factor that controls IDL RNA expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Transformation, Neoplastic / genetics*
  • DNA, Mitochondrial / analysis
  • DNA, Mitochondrial / genetics*
  • Gene Expression Regulation*
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA / analysis
  • RNA / chemistry
  • RNA / isolation & purification*
  • RNA, Mitochondrial
  • Trans-Activators / physiology
  • Transcription, Genetic
  • Transcriptional Activation
  • Up-Regulation


  • DNA, Mitochondrial
  • RNA, Mitochondrial
  • Trans-Activators
  • RNA

Associated data

  • GENBANK/AF079515