Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis

Biochem Biophys Res Commun. 2000 Sep 24;276(2):461-5. doi: 10.1006/bbrc.2000.3503.


Bioglass 45S5 is an osteoproductive material, which resorbs by releasing its constitutive ions into solution. Treatment with the ionic products of Bioglass 45S5 dissolution in DMEM for 4 days increased human osteoblast proliferation to 155% of control. Two days after treatment, differential gene expression was analyzed by cDNA microarrays. Expression of a potent osteoblast mitogenic growth factor, insulin-like growth factor II (IGF-II), was increased to 290%. Additionally, there was a 168% increase in the concentration of unbound IGF-II protein in the conditioned media of treated osteoblasts. Expression levels of IGFBP-3, an IGF-II carrier protein, metalloproteinase-2 and cathepsin-D were also increased to 200, 340, and 310% of control levels, respectively. Metalloproteinase-2 and cathepsin-D are proteases that cleave IGF-II from its carrier proteins, resulting in the release of the unbound biologically active IGF-II. We suggest that the stimulatory effect of the ionic products of Bioglass 45S5 dissolution on osteoblast proliferation may be mediated by IGF-II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Substitutes / chemistry
  • Bone Substitutes / pharmacology
  • Cell Division / drug effects
  • Cells, Cultured
  • Ceramics / chemistry
  • Ceramics / pharmacology*
  • DNA, Complementary / analysis
  • Gene Expression Profiling
  • Humans
  • Insulin-Like Growth Factor II / biosynthesis*
  • Insulin-Like Growth Factor II / genetics
  • Insulin-Like Growth Factor II / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction


  • Bioglass
  • Bone Substitutes
  • DNA, Complementary
  • RNA, Messenger
  • Insulin-Like Growth Factor II