Expression profiling of human sulfotransferase and sulfatase gene superfamilies in epithelial tissues and cultured cells

Biochem Biophys Res Commun. 2000 Oct 14;277(1):236-45. doi: 10.1006/bbrc.2000.3643.


The bioavailability of drugs administered topically or orally depends on their metabolism by epithelial enzymes such as the cytosolic sulfotransferases (SULT). Reverse transcriptase-polymerase chain reaction (RT-PCR) methods were established to detect expression of 8 SULT genes and 4 arylsulfatase (ARS) genes in human tissues of epithelial origin and in cultures of normal and transformed (cancer) cells. The results indicate: (i) SULT 1A1, 1A3, ARSC, and ARSD genes are ubiquitously expressed; (ii) expression is frequently similar between cell lines and corresponding tissues; (iii) SULT gene expression in normal cultured cells is generally comparable to the expression in associated transformed (cancer) cell lines; (iv) SULT 1A1 promoter usage is mainly tissue specific; however, both promoters are frequently used in SULT 1A3 expression; and (v) the expression profile of SULT 1A1, 1A3, 1E1, and 2B1a/b suggests that one or more of these isoforms may be involved in the cutaneous sulfoconjugation of minoxidil and cholesterol.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Availability
  • Cell Transformation, Neoplastic / genetics
  • Cells, Cultured
  • Cholesterol / metabolism
  • Cytoplasm / enzymology
  • Cytoplasm / metabolism
  • DNA Primers
  • Epithelial Cells / cytology
  • Epithelial Cells / enzymology*
  • Epithelial Cells / metabolism
  • Epithelium / enzymology*
  • Epithelium / metabolism
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Isoenzymes / genetics
  • Male
  • Minoxidil / metabolism
  • Neoplasm Proteins / genetics
  • Organ Specificity
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sulfatases / genetics*
  • Sulfotransferases / genetics*
  • Tumor Cells, Cultured


  • DNA Primers
  • Isoenzymes
  • Neoplasm Proteins
  • RNA, Messenger
  • Minoxidil
  • Cholesterol
  • Sulfotransferases
  • Sulfatases